Internal Dataset
Renal circadian rhythm dysregulation in lupus nephritis
UID: 10414
- Description
- This study examines the circadian gene expression of whole kidneys of young and nephritic NZB/W F1 mice. Young mice were harvested at 12 weeks of age. Nephritic mice were harvested 2-3 weeks after nephritis onset (>300mg/dl proteinuria) but before the development of endstage renal failure (BUN >25). Remission was induced in nephritic mice with new onset proteinuria of less than 1 week duration measured twice 24 hrs apart using one dose of cyclophosphamide 50mg/kg and 6 doses of CTLA4Ig (200ug) and anti-CD40L (250ug) given over 2 weeks. Kidneys were harvested 8 weeks later from mice still in remission with proteinuria 30mg/dl. Kidneys were perfused with PBS and total RNA was isolated from perfused mouse kidneys using TRIzol reagent
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Free to All
- Instructions
- The BioProject database provides an organizational framework to access information about research projects with links to data that have been or will be deposited into archival databases maintained at members of the International Nucleotide Sequence Database Consortium.
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- Grant Support
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Lupus Research Alliance/Lupus Research Alliance
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