Proteomics analyses of dexamethasone-treated PB and CB erythroid progenitors

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ZSOM Data Catalog

Internal Dataset

Proteomics analyses of dexamethasone-treated PB and CB erythroid progenitors

UID: 10369

Author(s): Ryan Ashley, Hongxia Yan, Nan Wang, John Hale, Brian Dulmovits... See more...

Julien Papoin, Meagan Olive, Namrata Udeshi, Steven Carr, Adrianna Vlachos, Jeffrey Lipton, Lydie Da Costa, Christopher Hillyer, Sandrina Kinet, Naomi Taylor, Narla Mohandas*, Anupama Narla*, Lionel Blanc*

* Corresponding Author

Description: Biological triplicates of peripheral blood (PB)- and cord blood (CB)-derived colony-forming unit-erythroids (CFU-Es) were treated in the absence or presence of dexamethasone (100 nM) for 24 hours. These cells were then processed for proteomics analysis by liquid chromatography–tandem mass spectrometry (LC-MS/MS). Proteomics quantified 10,045 proteins in PB samples and 10,028 proteins in CB samples. Proteomic evaluation of PB and CB samples was performed in 2 separate tandem mass tag–6 (TMT-6) plexes. This allowed for precise measurements of changes in protein abundance due to drug treatment in the PB-plex and the CB-plex, but less precise comparisons between PB and CB plexes.
Local Expert: Adrianna Vlachos

Jeffrey Lipton

Lionel Blanc

Subject of Study

Subject Domain

Chronic Disease

Drug Resistance

Population Age

Adult (19 years - 64 years)

Subject Gender

Male

Female

Keywords

Diamond-Blackfan Anemia

Erythroid Precursor Cells

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